A protocol for in vitro polyploidy induction was developed in Mini Red cultivar of African daisy Gerbera jamesonii. Various concentrations of colchicine 0, 0. Murashige and Skoog MS medium supplemented with 8. Root induction occurred when proliferated colchicine-treated shoots transferred to the half strength MS medium supplemented with 3. The results of analysis based on the comparison of means revealed that increasing concentrations of colchicine led to significant reduction in survival rate of Gerbera in longer exposure durations; however we achieved the highest survival rate with polyploidy induction by using 0. We obtained the highest percentage of tetraploid plants Colchicine-induced tetraploid plants of Gerbera represented reduced stomata density with wider size than their diploid relatives. In vitro tetraploidy induction led to larger flower size, longer stalks, broader leaf length and width and lower shoots in Mini Red cultivar of Gerbera. The optimized polyploidy induction protocol has high commercial value and can be also applicable for in vitro micropropagation of Gerbera. African daisy Gerbera jamesonii cv.
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